■ 基本信息
启动子: | hU6 |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒大小: | 4893bp |
质粒标签: | EGFP |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因沉默 |
片段类型: | shRNA
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片段物种: |
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原核抗性: | Kan |
真核抗性: | G418
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荧光标记: |
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■ 质粒简介
The pGenesil-1 plasmid was obtained by the transformation of pEGFP-C1 plasmid. PEGFP-C1 plasmid has been widely used in mammalian cells for many years, it has fluorescent protein expression, it is very convenient to detect the effect of. When your target gene was not detected by conventional fluorescence detection, the pGenesil-1 plasmid encoding shRNAs was a better choice.
■ 质粒图谱