■ 基本信息
启动子: | CMV |
复制子: | pUC |
质粒大小: | 7567bp |
原核抗性: | Amp |
真核抗性: | EGFP |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因沉默 |
片段类型: | shRNA
|
片段物种: |
|
原核抗性: | Amp |
真核抗性: |
|
荧光标记: | 绿色
|
■ 质粒简介
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
（pSico shRNA cloning protocol点击下载）
pSicoshRNAcloningprotocol.pdf
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK826pSicoR慢病毒干扰质粒.txt
