■ 基本信息
别名: | Plasmid #12251;pMDLg/pRRE |
启动子: | CMV |
复制子: | pUC |
终止子: | β-globin poly(A) |
质粒大小: | 8890bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 辅助包装 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
This 3rd generation LV packaging plasmid includes gag, coding for the virion main structural proteins; pol, responsible for the retrovirus-specific enzymes; and RRE, a binding site for the Rev protein which facilitates export of the RNA from the nucleus. This plasmid does not include Rev. The third generation packaging system offers maximal biosafety but is more cumbersome, as it involves the transfection of four different plasmids in the producer cells.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK841pMDLg-pRRE慢病毒包装质粒.txt
