■ 基本信息
启动子: | 35s |
复制子: | ori |
终止子: | NOS |
质粒分类: | 植物系列，蛋白过表达载体 |
质粒大小: | 12880bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
5'测序引物: | 35S:GACGCACAATCCCACTATCC |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Kan |
真核抗性: | G418
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荧光标记: |
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■ 质粒简介
An intron sequence of Poptr;CYCD1;1 from Populus trichocarpa was ligated into binary vector,pROKⅡ, to obtain the construct for gene silencing in plants. The resulting pRNAi was used to silence the exogenous fusion gene, GUS:GFP, in transgenic tobacco transformed with pCAMBIA-1303 vector. The specific sequence from GUS and GFP genes was cloned into pRNAi, respectively, and both of them reduced the expression of GUS:GFP gene. The results implied that the present pRNAi could be used to silence some gene of interest.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK848pROKII植物表达质粒.txt
