■基本信息
启动子: | 35s |
复制子: | ori |
终止子: | NOS |
质粒分类: | 植物系列，蛋白过表达载体 |
质粒大小: | 4530bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
5'测序引物: | 35S:GACGCACAATCCCACTATCC |
3'测序引物: | 根据序列设计引物 |
■质粒属性
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: | 绿色
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■质粒简介
Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters.
■质粒图谱
■质粒序列
质粒序列请下载：
ZK855pBI221-EGFP植物表达质粒.txt
