■ 基本信息
启动子: | 35s |
复制子: | pVS1V |
质粒分类: | 植物系列，蛋白过表达载体 |
原核抗性: | Kan |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 植物细胞 |
5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Kan |
真核抗性: | Hyg
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荧光标记: |
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■ 质粒简介
This vector contains a fully functional gusA reporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses E.coli gusA with an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual A.tumefaciens cells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the gusA gene and insert their own gene of interest in its place or use these vectors to create fusions of gusA with their gene of interest. These vectors contain the pUC18 polylinker-lacZa.
