■ 基本信息
启动子: | CaMV 35S promoter |
复制子: | pVS1V |
终止子: | NOS |
质粒大小: | 12361bp |
原核抗性: | Kan |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 植物 |
5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
■ 质粒属性
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Kan |
真核抗性: | Hyg
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荧光标记: |
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■ 质粒简介
Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the mgfp5:gusA fusion reporter genes.The pCambia vector backbone is derived from the pPZP vectors. The pCambia1304 vector offers:1.high copy number in E.coli for high DNA yield 2.pVS1 replicon for high stability in Agrobacterium 3.small size 4.restriction sites for modular plasmid modifications and small but adequate polylinkers for introducing DNA of interest 5.bacterial selection with kanamycin 6.plant selection with hygromycin B 7.simple means to construct translational fusions to mgfp5-gusA reporter genes.
■ 质粒图谱
■ 质粒序列
质粒序列请下载:ZK861pCAMBIA1304植物表达质粒.txt