■ 基本信息
启动子: | T7 |
复制子: | ori |
终止子: | T1,T2 |
质粒分类: | 植物系列，gateway载体 |
质粒大小: | 4762bp |
质粒标签: | ccdB |
原核抗性: | Kan |
克隆菌株: | DB3.1 |
培养条件: | 37度 |
表达宿主: | 植物细胞 |
5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
3'测序引物: | M13R:CAGGAAACAGCTATGACC |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因模板 |
片段类型: | ORF,GateWay |
片段物种: | 空载体 |
原核抗性: | Kan |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pDONR™ vectors are Gateway®-adapted vectors designed to generate attL-flanked entry clones containing your gene of interest following recombination with an attB expression clone or an attB PCR product. After creating an entry clone, your gene of interest may then be easily shuttled into a large selection of expression vectors using the Gateway® LR recombination reaction. Refer to the following table for a list of the available pDONR™ vectors.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK873pDONR221Kan大肠克隆质粒.txt
