■ 基本信息
启动子: | 35s |
复制子: | pUC |
终止子: | NOS |
质粒分类: | 植物系列，RNAi载体 |
质粒大小: | 12880bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 植物细胞 |
5'测序引物: | 35S:GACGCACAATCCCACTATCC |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 植物 |
质粒用途: | 基因沉默 |
片段类型: | shRNA |
片段物种: |
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原核抗性: | Kan |
真核抗性: | G418
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荧光标记: |
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■ 质粒简介
siRNA) caused by RNA (interference RNA short)Interference (RNAi) is a very effective test method. It is a creature The body of a molecule by double stranded RNA (dsRNA) molecules to make specific Degradation of the sequence of mRNA resulted in the silencing of gene expression. This phenomenon occurs at the post transcriptional level, that is, sequence specific Post transcriptional gene silencing.
