■ 基本信息
启动子: | AOX1 |
复制子: | pUC |
终止子: | CYC1 |
质粒分类: | 酵母系列,毕赤酵母表达载体 |
质粒大小: | 7898bp |
原核抗性: | Amp |
真核抗性: | ADE2 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母细胞 |
5'测序引物: | AOX5:GACTGGTTCCAATTGACAAGC |
3'测序引物: | AOX3:GGCAAATGGCATTCTGACAT |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System. The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains. The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted. You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit.
■ 质粒图谱
■ 质粒序列
质粒序列请下载:ZK934pPinkα-HC毕赤酵母质粒.txt