■ 基本信息
启动子: | GAL1 |
复制子: | 2μFI |
终止子: | CYC1 |
质粒大小: | 5963bp |
原核抗性: | Amp |
真核抗性: | URA3 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母细胞 |
5'测序引物: | GAL1-F:ATTTTCGGTTTGTATTACTTC |
3'测序引物: | GALI-R:GTTCTTAATACTAACATAACT |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | URA3
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荧光标记: |
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■ 质粒简介
The pYC2/CT vector contains the CEN6/ARSH4 sequence (Sikorski and Hieter, 1989) for maintenance and replication in yeast. The CEN6/ARSH4 sequence is a 518 bp hybrid DNA fragment that contains a yeast centromere sequence (CEN) and an autonomously replicating sequence (ARS) (Sikorski and Hieter, 1989). The CEN6 sequence is derived from the CEN6 locus of yeast chromosome 6 (Panzeri and Philippsen, 1982) while the ARSH4 sequence is derived from the yeast histone H4-associated ARS (Bouton and Smith, 1986). When placed in a heterologous expression plasmid (i.e. pYC2/CT), the presence of the CEN6/ARSH4 sequence allows non-integrative centromeric maintenance and low copy number replication of the plasmid (generally 1–2 copies per cell).
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK950pYES2CT酿酒酵母质粒.txt
