■ 基本信息
启动子: | GAL1 |
复制子: | 2μFI |
终止子: | CYC1 |
质粒分类: | 酵母系列，酿酒酵母表达载体 |
质粒大小: | 5870bp |
原核抗性: | Amp |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母细胞 |
诱导方式: | 半乳糖诱导 |
5'测序引物:
| GAL1-F:ATTTTCGGTTTGTATTACTTC
|
3'测序引物: | GALI-R:GTTCTTAATACTAACATAACT |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | TRP1
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荧光标记: |
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■ 质粒简介
pYES3/CT and pYES6/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Vectors feature a C-terminal V5 epitope for detection with an Anti-V5 Antibody. pYES3/CT carries the TRP1 selection marker for selection in yeast. pYES6/CT carries the bsd resistance gene for selection with the antibiotic Blasticidin, a potent selection agent that can be used at very low concentrations and in any strain regardless of auxotrophic marker.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK952pYES3CT酿酒酵母质粒.txt
