■ 基本信息
启动子: | TRP1，T3,T7 |
复制子: | pUC |
质粒分类: | 酵母系列，毕赤酵母表达载体 |
质粒大小: | 4783bp |
原核抗性: | Amp |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母细胞 |
诱导方式: | 半乳糖诱导 |
5'测序引物: | M13R:CAGGAAACAGCTATGACC |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: |
|
片段物种: |
|
原核抗性: | Amp |
真核抗性: | TRP1
|
荧光标记: |
|
■ 质粒简介
YCtype centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ) peptide. pRSS56, constructed by ligating a PvuI fragment (bp 4982412) of pBluescript KS+ to a PvuI fragment (bp 2850 730) of pBS(+), contains the KS MCS from pBluescript KS(+) and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A genomic HindII/PstI fragment (1.002 kb) containing the TRP1 gene was inserted into the NdeI site and a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted. An EcoRI site in the TRP1containing fragment (external to the coding sequence) was destroyed. The order of the major features in this plasmid is: TRP1f1 ori (NaeI) T7 promoter lacZ/MCS T3 promoter pMB1 ori bla CEN6 ARSH4.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK956pRS314酿酒酵母质粒.txt
