■ 基本信息
复制子: | pUC |
质粒分类: | 酵母系列质粒；酵母表达质粒；酿酒酵母质粒 |
质粒大小: | 4456bp |
原核抗性: | Amp |
真核抗性: | HIS3 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | YPH499，YPH500等酿酒酵母 |
培养条件: | 30℃，YPD，有氧 |
5'测序引物: | T7(TAATACGACTCACTATAGGG) |
3'测序引物: | M13R(CAGGAAACAGCTATGACC) |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | HIS3
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荧光标记: |
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■ 质粒简介
pRS4xx系列质粒被用于基因的穿梭载体克隆酿酒酵母，但能够在大肠杆菌中复制。虽然在大肠杆菌中，他们从pMB1的复制原点复制至pBR322。他们携带的bla（APR）标记用于选择用氨苄青霉素和复制的噬菌体F1原点单链DNA 。pRS403 through pRS406 are integrative plasmids (YIp); pRS413through pRS416 (shown below) are centromeric plasmids (YCp),and differ from the YIp by the insertion of a centromere (CEN)and autonomously replicating sequence (ARS); pRS423 throughpRS426 are episomal plasmids (YEp), and carry instead theorigin of replication from the yeast 2µ circle plasmid along withREP3 and FRT sequences (2). The 4 vectors in each series differonly in the yeast auxotrophic marker used: HIS3 (pRS4x3), TRP1(pRS4x4), LEU2 (pRS4x5) or URA3 (pRS4x6).
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK959pRS403酿酒酵母质粒.txt
