■ 基本信息
启动子: | URA3，T7,T3 |
复制子: | 2μFI |
质粒分类: | 酵母系列，酿酒酵母表达载体 |
质粒大小: | 5726bp |
原核抗性: | Amp |
真核抗性: | URA3 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母细胞 |
诱导方式: | 半乳糖诱导 |
5'测序引物: | M13R:CAGGAAACAGCTATGACC |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | URA3
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荧光标记: |
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■ 质粒简介
The order of the major features in this plasmid is:ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori (2 micron) - URA3. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 + or - 1.4% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PstI--4.0, 1.7; PvuII--5.2, 0.54. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416322 Hosts: E.coli DH5alpha, Saccharomyces cerevisiae, E.coli.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK963pRS426酿酒酵母质粒.txt
