■ 基本信息
别名: | pGADT7-AD |
启动子: | ADH1 |
复制子: | 2μ |
质粒分类: | 酵母系列，酵母双杂交载体 |
质粒大小: | 7987bp |
原核抗性: | Amp |
真核抗性: | LEU2 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母细胞 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物:
| 根据序列设计引物
|
备注: | 酵母双杂交空载体 |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | LEU2
|
荧光标记: |
|
■ 质粒简介
pGADT7 AD is a yeast expression vector that is designed to express a protein of interestfused to a GAL4 activation domain (AD; amino acids 768–881). Transcription of the GAL4 ADfusion is driven by the constitutively active ADH1 promoter (PADH1), and is terminated at theADH1 transcription termination signal (TADH1). The GAL4 AD fusion contains an N-terminalSV40 nuclear localization signal (SV40 NLS; 1) that targets the protein to the yeast nucleus,and a hemagglutinin epitope tag (HA Tag), located between the GAL4 AD and the proteinof interest, that allows the protein to be easily detected with HA-tag antibodies.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK974pGADT7（别名pGADT7-AD）酵母双杂交质粒.txt
