■ 基本信息
启动子: | ADH1 |
复制子: | 2μ |
质粒分类: | 酵母系列，酵母双杂交载体 |
质粒大小: | 8.3kb |
原核抗性: | Kan |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母细胞 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: |
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片段物种: |
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原核抗性: | Kan |
真核抗性: | TRP1
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荧光标记: |
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■ 质粒简介
Yeast two-hybrid systems provide a sensitive method for detecting relatively weak and transient protein interactions. Such interactions may not be biochemically detectable, but may be critical for proper functioning of complex biological systems. pGBKT7-53 and pGADT7-T encode fusions between the GAL4 DNA-BD and AD and murine p53 and SV40 large T-antigen, respectively. p53 and large T-antigen interact in a yeast two-hybrid assay.
■ 质粒图谱
■ 质粒序列
质粒序列可参考（具体以测序为准）下载：
ZK981 pGBKT7-53酵母双杂交质粒.txt
