■ 基本信息
启动子: | CYC1 promoter |
复制子: | pUC ori，F1 ori，2μ ori |
终止子: | CYC1 terminator |
质粒分类: | 酵母细胞质粒；酵母杂交质粒；双杂交类质粒 |
质粒大小: | 6200bp |
质粒标签: | N-NubG；N-HA |
原核抗性: | Amp |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母菌 |
培养条件: | 30℃，YPD，有氧 |
5'测序引物: | CYC1-F：CTTTCCTTATACATTAGGACC |
3'测序引物:
| CYC1-R：GGGACCTAGACTTCAGGTTG |
备注: | 高拷贝质粒 |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | TRP1
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荧光标记: |
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■ 质粒简介
pPR3-N是一个用于膜蛋白酵母双杂交实验的质粒。The DUALmembrane system is intended for the detection and identification of interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane pairwise interaction kit enables you to:
Investigate the interaction between a membrane protein (integral or membrane-associated) and a membrane protein or soluble protein
Map domains or amino acids which are critical for an interaction
Screen cDNA libraries using a membrane protein as a bait to find novel
interacting proteins (requires purchase of a separate cDNA library from Dualsystems)
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK987pPR3-N膜蛋白双杂交质粒.txt
