■ 基本信息
启动子: | P43 |
质粒分类: | 广宿主系列,枯草杆菌载体 |
质粒大小: | 3787bp |
原核抗性: | Kan |
克隆菌株: | BS168 |
培养条件: | 37度 |
表达宿主: | 枯草芽孢杆菌 |
5'测序引物: | 根据序列设计引物 |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 枯草杆菌 |
质粒用途: | 蛋白表达,分泌表达 |
片段类型: | ORF |
片段物种: | 空载体
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原核抗性: | Kan |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pWB705 andpWB980 . pUB110 is the parental plasmid for pUB18 and its derivatives.pWB705 is a pUB18-based secretion vector carrying both the constitutive expressed P43 promoter and the engineered levansucrase signal sequence. pWB980 is a pUB19-based secretion vector. The transcription direction of the P43 promoter is the same as that for the majority of the pUB110-encoded genes. rep, kan, ble, mob and sacB SP represent thesequence coding for replicase, kanamycin resistance marker,bleomycin resistance marker, mobilization protein and the levansucrase signal sequence, respectively. The arrows show the transcription directions for these genes.
■ 质粒图谱
■ 质粒序列
质粒序列可参考(具体以测序为准)下载:ZK1024pWB980枯草分泌质粒.txt
质粒只保证关键序列正确,不保证表达效果。