■ 基本信息
启动子: | Ac5 |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 昆虫系列质粒；昆虫游离质粒；昆虫胞内质粒 |
质粒大小: | 6118bp |
质粒标签: | N-EGFP，C-V5，C-6×His |
原核抗性: | Amp |
真核抗性: | 无 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 果蝇细胞系S2 |
诱导方式: | 无需诱导，组成型表达 |
5'测序引物: | Ac5-F(ACACAAAGCCGCTCCATCAG) |
3'测序引物:
| BGH-R(TAGAAGGCACAGTCGAGG)
|
备注: | 非病毒 |
■ 质粒属性
质粒宿主: | 昆虫细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: |
|
荧光标记: | 绿色
|
■ 质粒简介
pAC5.1b-EGFP-2质粒是一个昆虫细胞组成型表达载体，Ac5启动子驱动EGFP标签、目的基因、Myc标签和6×HIS标签一起融合表达，可以与筛选载体pCoHygro或者pCoBlast共转染，建立稳表达细胞系。
pAC5.1b-EGFP② is a 6kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, pAC5.1b-EGFP allows selection of stable cell lines exhibiting constitutive expression of the protein of interest.
• The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells (Chung and Keller, 1990).
• Multiple cloning site to facilitate cloning the gene of interest.
• C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest (if desired).
• Three reading frames to facilitate in-frame cloning with the C-terminal peptide.
• N-terminal EGFP tag for better detection.
• Ampicillin resistance gene for selection of transformants in E. coli.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1035pAC5.1b-EGFP-2昆虫胞内质粒.txt
质粒只保证关键序列正确，不保证表达效果。
