■ 基本信息
启动子: | PH |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 昆虫细胞载体；杆状病毒组成型表达质粒 |
质粒大小: | 5463bp |
质粒标签: | C-3C，C-GST |
原核抗性: | Amp |
真核抗性: | 庆大霉素Gentamicin |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 昆虫细胞 |
诱导方式: | 组成型表达，无需诱导 |
5'测序引物: | pFastbac-F:TATTCCGGATTATTCATACC |
3'测序引物:
| pFastBac-R:ACAAATGTGGTATGGCTGA |
备注: | 可表达GST融合蛋白 |
■ 质粒属性
质粒宿主: | 昆虫细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
The Bac-to-Bac® Baculovirus Expression System provides a rapid and efficient method to generate recombinant baculoviruses (Ciccarone et al., 1997). This method was developed by researchers at Monsanto, and is based on site-specific transposition of an expression cassette into a baculovirus shuttle vector (bacmid) propagated in E. coli (Luckow et al., 1993). The major components of the Bac-to-Bac® Baculovirus Expression System include:
• A choice of pFastBac™ donor plasmids that allow generation of an expression construct containing the gene of interest where expression of the gene of interest is controlled by a baculovirus-specific promoter.
• An E. coli host strain, DH10Bac™, that contains a baculovirus shuttle vector (bacmid) and a helper plasmid, and allows generation of a recombinant bacmid following transposition of the pFastBac expression construct.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1040pFastBac-GST-N昆虫胞内质粒.txt
质粒只保证关键序列正确，不保证表达效果。
