■ 基本信息

■ 质粒属性

■ 质粒简介

pFastBac Dual昆虫细胞表达载体包含有两个启动子，能够同时表达两个外源基因，一个表达基因是polyhedrin (PH)启动子控制，另一个外源基因是由p10启动子控制。

pFastBacDual 载体的特点是在单个载体上有两个启动子，用于在使用 Bac-to-Bac® 杆状病毒表达系统(Bac-to-Bac® Baculovirus Expression System) 时在昆虫细胞中同时表达两个蛋白质。该载体具有两个强启动子，多角体蛋白启动子和p10 启动子，因此可实现高水平表达。包含 CAT和β-葡萄糖醛酸酶 (gus) 的表达对照。

The Bac-to-Bac® Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac® Baculovirus Expression System , which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:

Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.

Easy colony screening. The parent bacmid in DH10Bac™ E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.

■ 质粒图谱

■ 质粒序列

质粒序列请下载：ZK1045pFastBacDual昆虫双框质粒.txt

质粒只保证关键序列正确，不保证表达效果。