■ 基本信息

■ 质粒属性

■ 质粒简介

pLI50质粒是一个金黄色葡萄球菌单拷贝整合载体，在大肠杆菌里氨苄抗性，在金黄色葡萄球菌里是氯霉素抗性。参考文献：Construction of single-copy integration vectors for Staphylococcus aureus.

Single-copy integration vectors suitable for cloning in Staphylococcus aureus have been constructed. Their construction was based on the site-specific recombination system of staphylococcal phage, L54a. The vectors are capable of autonomous replication in Escherichia coli, but they are not endowed with a replication function in S. aureus. As a consequence, establishment of these vectors in S. aureus can only be achieved by the integration system of the phage. Once integrated into the chromosome, the vectors, or their derivatives, were stably inherited even without selective pressure. Because such a vector exists in an integrated form in S. aureus, the gene dosage of the DNA cloned in the vector matches that of the chromosome.

■ 质粒图谱

■ 质粒序列

质粒序列请下载：ZK1134pLI50金黄色葡萄球菌单拷贝整合质粒.txt

质粒只保证关键序列正确，不保证表达效果。