■ 基本信息
别名: | 四环素激活 |
启动子: | Tet |
复制子: | pUC |
质粒分类: | 慢病毒表达质粒 |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
5'测序引物: | 根据全序列设计 |
3'测序引物: | 根据全序列设计 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: |
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原核抗性: | Amp |
真核抗性: | Puro
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荧光标记: |
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■ 质粒简介
慢病毒载体pLVX-TetOne-Puro是集调控与应答功能于一体的四环素诱导载体。The Lenti-X Tet-One Inducible Expression System (Puro) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing and non-dividing mammalian cells. The all-in-one pLVX-TetOne-Puro Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation and your gene of interest from the PTRE3GS promoter in the reverse orientation. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. In addition, this vector contains a puromycin antibiotic selection marker.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1692 pLVX-TetOne-Puro慢病毒四环素激活表达质粒.txt
