■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal,hGH poly(A) signal |
质粒分类: | 哺乳系列质粒；哺乳表达质粒；pCMV系列质粒 |
质粒大小: | 6299bp |
质粒标签: | N-3×FLAG |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 293T等哺乳细胞 |
培养条件: | 37℃，5%CO2 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物:
| CMV-F（CGCAAATGGGCGGTAGGCGTG） |
3'测序引物: | hGH-PA-R（CCAGCTTGGTTCCCAATAGA） |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | G418
|
荧光标记: |
|
■ 质粒简介
p3×FLAG-CMV-10表达载体是用于在哺乳动物细胞中建立N-3×FLAG融合蛋白的瞬时或稳定细胞内的pCMV5表达载体的升级版。该载体在多克隆区域上游编码三个相邻的FLAG表位（Asp-Tyr-Lys-Xaa-Xaa-Asp）。这导致使用FLAG抗体的检测灵敏度增加。第三个FLAG表位包括肠激酶识别序列，允许从纯化的融合蛋白切割3×FLAG肽。
The p3×FLAG-CMV-10 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG® epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
p3×FLAG-CMV-10 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigenexpressing
host, such as COS cells.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1697 p3×Flag-CMV-10哺乳表达质粒.txt
