■ 基本信息
启动子: | H1 promoter |
复制子: | pBR322 ori，SV40 ori |
质粒大小: | 4555bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因沉默 |
片段类型: | shRNA
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片段物种: |
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原核抗性: | Amp |
真核抗性: | Hyg
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荧光标记: |
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■ 质粒简介
pSilencer3.1-H1-Stuffer-Hygro是一个哺乳细胞干扰载体，通过BamHI和HindIII酶切位点可连入SiRNA，点击查看本质粒的参考资料：
pSilencer hygro Kit.pdf.（点击查看）
The pSilencer vectors employ RNA polymerase III (pol III) promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1717 pSilencer3.1-H1-Stuffer-Hygro哺乳干扰质粒.txt
