■ 基本信息
原核抗性: | Amp |
真核抗性: | HIS3 |
| 克隆菌株: | DH5a |
培养条件: | 37度 |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | HIS3
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荧光标记: |
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■ 质粒简介
pGilda is a cloning vector used to generate fusions of a bait protein with the 202-residue LexA protein, which acts as a DNA-binding domain in the MATCHMAKER LexA Two-Hybrid System . The hybrid protein is expressed in yeast cells from the tightly regulated GAL1 promoter (PGAL1); transcription is repressed in the presence of glucose and induced in the the absence of glucose and presence of galactose . pGilda carries the HIS3 gene for selection in His– auxotrophic yeast strains and is compatible with MATCHMAKER LexA Libraries. This vector replicates autonomously in both E. coli (using the Col E1 ori) and in S. cerevisiae (using the CEN/ARS ori). Ampr is used for selection in E. coli.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1778 pGilda酵母双杂交质粒.txt
