■ 基本信息
复制子: | PUC |
原核抗性: | Amp |
真核抗性: | 绿色荧光蛋白GFPuv |
克隆菌株: | DH5a |
培养条件: | 37度 |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: | 绿色
|
■ 质粒简介
pGFPuv是一个大肠杆菌绿色荧光表达质粒。pGFPuv carries the“cycle 3”variant of GFP described by Crameri et al. (1). This gene was cloned between the two MCSs of the pUC19 derivative pPD16.43 (2). The GFPuv gene canbe easily excised from pGFPuv. Alternatively, the GFPuv coding sequence can be amplified by PCR. The GFPuv gene was inserted in frame with the lacZ initiation codon from pUC19 so that a β-galactosidase-GFPuv fusionprotein is expressed from the lac promoter in E. coli . Note, however, that if you excise the GFPuv coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode the native (i.e.,non-fusion) GFPuv protein. The pUC backbone of pGFPuv provides a high copy number origin of replication and ampicillin resistance gene for propagation in E. coli.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1842 pGFPuv大肠荧光质粒.txt
