■ 基本信息
别名: | 金黄葡萄球染体异位互补 |
原核抗性: | Amp |
| 克隆菌株: | DH5a |
培养条件: | 37度 |
■ 质粒属性
质粒宿主: | 金黄色葡萄球菌 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureu.pECTO a Gram negative/Gram positive shuttle vector derived from pMAD2 designed to integrate DNA sequences between SAOUHSC_00278 and SAOUHSC_00279 (NCTC8325 nomenclature HG003). It carries about 1kb of both sequences. A terminator introduced between the SAOUHSC_00278 and SAOUHSC_00279 sequences prevents polar effects associated with the expression of inserted genes.This a Gram negative/Gram positive shuttle vector with two specialized replicons and antibiotic markers. pBR322 replicon and ampicillin resistance for E. coli. pE194(ts) replicon and erythromycin resistance for S. aureus. In S. aureus, the plasmid propagates at T<30°C, it becomes a suicide vector at T>37°C.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1904 pECTO金黄色葡萄球菌染色体基因异位互补质粒.txt
