■ 基本信息
别名: | Plasmid #79873;pAX01-dCas9 |
原核抗性: | Amp |
真核抗性: | Ery |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 枯草杆菌 |
■ 质粒属性
质粒宿主: | 枯草杆菌 |
质粒用途: | 基因编辑 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pJMP1质粒是一个枯草杆菌基因编辑质粒。Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA。dcas9 was PCR-amplified from pdCas9-bacteria (Addgene #44249) using primers containing BamHI-compatible BsaI sites, digested with BsaI, then ligated into plasmid pAX01 digested with BamHI to generate pJMP1。
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.
Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA
Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. PubMed Journal
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK2006 pJMP1枯草编辑质粒.txt
