产品名称：pEYFP-C1

货号	规格	价格	订购数量	是否现货
ZK2264	1μg(20μl,50ng/μl)	580	- +	有货

产品介绍

■ 基本信息

启动子:	CMV
复制子:	pUC ori,f1 ori
终止子:	SV40 poly(A) signal
质粒大小:	4731bp
原核抗性:	Kan
真核抗性:	Neo
克隆菌株:	DH5α
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	无须诱导，瞬时表达
5'测序引物:	pEGFP-C-5:CATGGTCCTGCTGGAGTTCGTG
3'测序引物:	Sv40-polyA-R：GAAATTTGTGATGCTATTGC

■ 质粒属性

质粒宿主:	哺乳细胞
质粒用途:	蛋白表达
片段类型:	ORF
片段物种:
原核抗性:	Kan
真核抗性:	Neo
荧光标记:

■ 质粒简介

pEYFP-C1 encodes an enhanced yellow-green variant of the Aequorea victoria green fluorescent protein (GFP). The EYFP gene contains the four amino acid substitutions previously published as GFP-10C (1): Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and and Thr-203 to Tyr. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 513-nm, the Em of EYFP is 36,500 cm1M1 and the fluorescent quantum yield is 0.63 (1), resulting in a bright fluorescent signal. The fluorescence observed is roughly equivalent to that from EGFP.

■ 质粒图谱

■ 质粒序列

质粒序列请下载：ZK2264pEYFP-C1哺乳荧光质粒.txt